LOCUS       62056_11695        6201 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             574..2223
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             2227..2346
                     /codon_start=1
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    complement(2398..2519)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2713..3070
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3101..3892
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3920..3968
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4295..4883)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5086..5943)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    6110..6201
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagtg ttttgcaagc aatcacaaga
       61 aagaatatga acgtctgcct tcccgtctct tctgcctgcc cctctccaaa ggtcacacta
      121 gttgtttttt cagcaccttg ggtgcatata gctcttaatt gtgattgtaa tagacctctg
      181 tcctttagag ctcattgttt taagcatgca gattgcatga ttcactaggt gccacttggg
      241 aaattgtggc agaatctcac aggagcaaag ctgtgccttg ttctgcttgc tttgttatat
      301 aaaactttat ttttaatgaa ttatttcata gtatattatc cttagaagca gtcattcagt
      361 gacagccatt ctccccatcc cagcctgtat taataaccag tcagagaaag tctaggagaa
      421 gtgggcatcc taatgaacat ttgtaattct gaccctgcat gagggccttt ggattagcaa
      481 gccctctgtc acctccttac tagagtaggg tgtgcctcac tgcgtctccg ttctttctaa
      541 gcttggcaat ccggtactgt tggtaaagcc accatggaag atgccaaaaa cattaagaag
      601 ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc
      661 atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg
      721 gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc
      781 tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc
      841 atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac
      901 aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc
      961 aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc
     1021 atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact
     1081 tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac
     1141 aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc
     1201 ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac
     1261 cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg
     1321 ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag
     1381 gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc
     1441 acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg
     1501 cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa
     1561 cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt
     1621 ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc
     1681 gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag
     1741 ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac
     1801 gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac
     1861 gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta
     1921 gccccagccg aactggagag catcctgctg caacacccca acatcttcga cgccggggtc
     1981 gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac
     2041 ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc
     2101 aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag
     2161 ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc
     2221 gtgaattctc acggcttccc tcccgaggtg gaggagcagg ccgccggcac cctgcccatg
     2281 agctgcgccc aggagagcgg catggataga caccctgctg cttgcgccag cgccaggatc
     2341 aacgtctaag gccgcgactc tagagtcggg gcggccggcc gcttcgagca gacatgataa
     2401 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     2461 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     2521 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     2581 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgttt gcgtattggg
     2641 cgctcttccg ctgatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
     2701 gttagctacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     2761 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2821 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2881 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     2941 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3001 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3061 cttttgcaaa aagctcgatt cttctgacac tagcgccacc atgatcgaac aagacggcct
     3121 ccatgctggc agtcccgcag cttgggtcga acgcttgttc gggtacgact gggcccagca
     3181 gaccatcgga tgtagcgatg cggccgtgtt ccgtctaagc gctcaaggcc ggcccgtgct
     3241 gttcgtgaag accgacctga gcggcgccct gaacgagctt caagacgagg ctgcccgcct
     3301 gagctggctg gccaccaccg gcgtaccctg cgccgctgtg ttggatgttg tgaccgaagc
     3361 cggccgggac tggctgctgc tgggcgaggt ccctggccag gatctgctga gcagccacct
     3421 tgcccccgct gagaaggttt ctatcatggc cgatgcaatg cggcgcctgc acaccctgga
     3481 ccccgctacc tgccccttcg accaccaggc taagcatcgg atcgagcgtg ctcggacccg
     3541 catggaggcc ggcctggtgg accaggacga cctggacgag gagcatcagg gcctggcccc
     3601 cgctgaactg ttcgcccgac tgaaagcccg catgccggac ggtgaggacc tggttgtcac
     3661 acacggagat gcctgcctcc ctaacatcat ggtcgagaat ggccgcttct ccggcttcat
     3721 cgactgcggt cgcctaggag ttgccgaccg ctaccaggac atcgccctgg ccacccgcga
     3781 catcgctgag gagcttggcg gcgagtgggc cgaccgcttc ttagtcttgt acggcatcgc
     3841 agctcccgac agccagcgca tcgccttcta ccgcttgctc gacgagttct tttaatgatc
     3901 tagaaccggt catggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     3961 tttgtgtgtt cgaactagat gctgtcgacc gatgcccttg agagccttca acccagtcag
     4021 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     4081 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
     4141 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     4201 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4261 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4321 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4381 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4441 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4501 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4561 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4621 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4681 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     4741 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4801 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4861 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4921 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4981 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5041 cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt gcttgatcag
     5101 tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct gactccccgt
     5161 cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag caatgatgcc
     5221 gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag cagggagggc
     5281 cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga gctgctgtcg
     5341 tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg ccattgctac
     5401 tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg gttcccagcg
     5461 gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct ccttagggcc
     5521 tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa tggcagcact
     5581 acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg gcgagtactc
     5641 aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc cggcgtctat
     5701 acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg ggaatcgttc
     5761 ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga tatagcccac
     5821 tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa
     5881 aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat gttggatgct
     5941 catactcgtc ctttttcaat attattgaag catttatcag ggttactagt acgtctctca
     6001 aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat aaaatatctt
     6061 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
     6121 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
     6181 caggtgccag aacatttctc t
//
